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Quorum quenching Bacillus sonorensis isolated from soya sauce fermentation brine.

ABSTRACT: An N-acylhomoserine lactone (AHL)-degrading bacterial strain, L62, was isolated from a sample of fermentation brine of Chinese soya sauce by using rich medium agar supplemented with soya sauce (10% v/v). L62, a rod-shaped Gram positive bacterium with amylolytic activity, was phylogentically related to Bacillus sonorensis by 16S ribosomal DNA and rpoB sequence analyses. B. sonorensis L62 efficiently degraded N-3-oxohexanoyl homoserine lactone and N-octanoylhomoserine lactone. However, the aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of AHLs, was not detected in L62, suggesting the presence of a different AHL-degrading gene in L62. To the best of our knowledge, this is the first report of AHL-degrading B. sonorensis from soya sauce liquid state fermentation.

SUBMITTER: Yin WF

PROVIDER: S-EPMC3355399 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Quorum quenching Bacillus sonorensis isolated from soya sauce fermentation brine.

Yin Wai-Fong WF Tung Hun-Jiat HJ Sam Choon-Kook CK Koh Chong-Lek CL Chan Kok-Gan KG

Sensors (Basel, Switzerland) 20120327 4

An N-acylhomoserine lactone (AHL)-degrading bacterial strain, L62, was isolated from a sample of fermentation brine of Chinese soya sauce by using rich medium agar supplemented with soya sauce (10% v/v). L62, a rod-shaped Gram positive bacterium with amylolytic activity, was phylogentically related to Bacillus sonorensis by 16S ribosomal DNA and rpoB sequence analyses. B. sonorensis L62 efficiently degraded N-3-oxohexanoyl homoserine lactone and N-octanoylhomoserine lactone. However, the aiiA ho ...[more]

PMID: 22666018

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