Ontology highlight
ABSTRACT:
SUBMITTER: Ishii J
PROVIDER: S-EPMC3356411 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Ishii Jun J Yoshimoto Nobuo N Tatematsu Kenji K Kuroda Shun'ichi S Ogino Chiaki C Fukuda Hideki H Kondo Akihiko A
PloS one 20120518 5
G-protein-coupled receptors (GPCRs) regulate a wide variety of physiological processes and are important pharmaceutical targets for drug discovery. Here, we describe a unique concept based on yeast cell-surface display technology to selectively track eligible peptides with agonistic activity for human GPCRs (Cell Wall Trapping of Autocrine Peptides (CWTrAP) strategy). In our strategy, individual recombinant yeast cells are able to report autocrine-positive activity for human GPCRs by expressing ...[more]