Ontology highlight
ABSTRACT:
SUBMITTER: Papapetrou EP
PROVIDER: S-EPMC3356916 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Papapetrou Eirini P EP Lee Gabsang G Malani Nirav N Setty Manu M Riviere Isabelle I Tirunagari Laxmi M S LM Kadota Kyuichi K Roth Shoshannah L SL Giardina Patricia P Viale Agnes A Leslie Christina C Bushman Frederic D FD Studer Lorenz L Sadelain Michel M
Nature biotechnology 20101212 1
Realizing the therapeutic potential of human induced pluripotent stem (iPS) cells will require robust, precise and safe strategies for genetic modification, as cell therapies that rely on randomly integrated transgenes pose oncogenic risks. Here we describe a strategy to genetically modify human iPS cells at 'safe harbor' sites in the genome, which fulfill five criteria based on their position relative to contiguous coding genes, microRNAs and ultraconserved regions. We demonstrate that ∼10% of ...[more]