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Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells.


ABSTRACT: We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation profiles and differentiation potential. Extended passage of some iPSC clones in culture did not improve their epigenetic resemblance to embryonic stem cells, implying that some human iPSCs retain a residual 'epigenetic memory' of their tissue of origin.

SUBMITTER: Kim K 

PROVIDER: S-EPMC3357310 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

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Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells.

Kim Kitai K   Zhao Rui R   Doi Akiko A   Ng Kitwa K   Unternaehrer Juli J   Cahan Patrick P   Huo Hongguang H   Loh Yuin-Han YH   Aryee Martin J MJ   Lensch M William MW   Li Hu H   Collins James J JJ   Feinberg Andrew P AP   Daley George Q GQ  

Nature biotechnology 20111127 12


We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation profiles and differentiation potential. Extended passage of some iPSC clones in culture did not improve their epigenetic resemblance to embryonic stem cells, implying that some huma  ...[more]

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