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Multiplexed nanoflares: mRNA detection in live cells.

ABSTRACT: We report the development of the multiplexed nanoflare, a nanoparticle agent that is capable of simultaneously detecting two distinct mRNA targets inside a living cell. These probes are spherical nucleic acid (SNA) gold nanoparticle (Au NP) conjugates consisting of densely packed and highly oriented oligonucleotide sequences, many of which are hybridized to a reporter with a distinct fluorophore label and each complementary to its corresponding mRNA target. When multiplexed nanoflares are exposed to their targets, they provide a sequence specific signal in both extra- and intracellular environments. Importantly, one of the targets can be used as an internal control, improving detection by accounting for cell-to-cell variations in nanoparticle uptake and background. Compared to single-component nanoflares, these structures allow one to determine more precisely relative mRNA levels in individual cells, improving cell sorting and quantification.

SUBMITTER: Prigodich AE

PROVIDER: S-EPMC3357311 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

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Multiplexed nanoflares: mRNA detection in live cells.

Prigodich Andrew E AE Randeria Pratik S PS Briley William E WE Kim Nathaniel J NJ Daniel Weston L WL Giljohann David A DA Mirkin Chad A CA

Analytical chemistry 20120130 4

We report the development of the multiplexed nanoflare, a nanoparticle agent that is capable of simultaneously detecting two distinct mRNA targets inside a living cell. These probes are spherical nucleic acid (SNA) gold nanoparticle (Au NP) conjugates consisting of densely packed and highly oriented oligonucleotide sequences, many of which are hybridized to a reporter with a distinct fluorophore label and each complementary to its corresponding mRNA target. When multiplexed nanoflares are expose ...[more]

PMID: 22288418

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