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Reduced levels of class 1 reversibly glycosylated polypeptide increase intercellular transport via plasmodesmata.

ABSTRACT: Maize and Arabidopsis thaliana class 1 reversibly glycosylated polypeptides (C1RGPs) are plasmodesmata-associated proteins. Previously, over-expression of Arabidopsis C1RGP AtRGP2 in Nicotiana tabacum was shown to reduce intercellular transport of photoassimilate, resulting in stunted, chlorotic plants, and inhibition of local cell-to-cell spread of tobacco mosaic virus (TMV). Here, we used virus induced gene silencing to examine the effects of reduced levels of C1RGPs in Nicotiana benthamiana. Silenced plants show wild-type growth and development. Intercellular transport in silenced plants was probed using fluorescently labeled TMV and its movement protein, P30. P30 shows increased cell-to-cell movement and TMV exhibited accelerated systemic spread compared to control plants. These results support the hypothesis that C1RGPs act to regulate intercellular transport via plasmodesmata.

SUBMITTER: Burch-Smith TM

PROVIDER: S-EPMC3357371 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature

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Reduced levels of class 1 reversibly glycosylated polypeptide increase intercellular transport via plasmodesmata.

Burch-Smith Tessa M TM Cui Ya Y Zambryski Patricia C PC

Plant signaling & behavior 20120101 1

Maize and Arabidopsis thaliana class 1 reversibly glycosylated polypeptides (C1RGPs) are plasmodesmata-associated proteins. Previously, over-expression of Arabidopsis C1RGP AtRGP2 in Nicotiana tabacum was shown to reduce intercellular transport of photoassimilate, resulting in stunted, chlorotic plants, and inhibition of local cell-to-cell spread of tobacco mosaic virus (TMV). Here, we used virus induced gene silencing to examine the effects of reduced levels of C1RGPs in Nicotiana benthamiana. ...[more]

PMID: 22274744

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