The lung tissue microbiome in chronic obstructive pulmonary disease.

ABSTRACT:

Rationale

Based on surface brushings and bronchoalveolar lavage fluid, Hilty and coworkers demonstrated microbiomes in the human lung characteristic of asthma and chronic obstructive pulmonary disease (COPD), which have now been confirmed by others.

Objectives

To extend these findings to human lung tissue samples.

Methods

DNA from lung tissue samples was obtained from nonsmokers (n = 8); smokers without COPD (n = 8); patients with very severe COPD (Global Initiative for COPD [GOLD] 4) (n = 8); and patients with cystic fibrosis (CF) (n = 8). The latter served as a positive control, with sterile water as a negative control. All bacterial community analyses were based on polymerase chain reaction amplifying 16S rRNA gene fragments. Total bacterial populations were measur

SUBMITTER: Sze MA 

PROVIDER: S-EPMC3359894 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature