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Simplified Insertion of Transgenes Onto Balancer Chromosomes via Recombinase-Mediated Cassette Exchange.


ABSTRACT: Balancer chromosomes are critical tools for Drosophila genetics. Many useful transgenes are inserted onto balancers using a random and inefficient process. Here we describe balancer chromosomes that can be directly targeted with transgenes of interest via recombinase-mediated cassette exchange (RMCE).

SUBMITTER: Sun FF 

PROVIDER: S-EPMC3362938 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

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Simplified Insertion of Transgenes Onto Balancer Chromosomes via Recombinase-Mediated Cassette Exchange.

Sun Florence F FF   Johnson Justine E JE   Zeidler Martin P MP   Bateman Jack R JR  

G3 (Bethesda, Md.) 20120501 5


Balancer chromosomes are critical tools for Drosophila genetics. Many useful transgenes are inserted onto balancers using a random and inefficient process. Here we describe balancer chromosomes that can be directly targeted with transgenes of interest via recombinase-mediated cassette exchange (RMCE). ...[more]

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