Ontology highlight
ABSTRACT:
SUBMITTER: Rajani DK
PROVIDER: S-EPMC3365155 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Rajani Danielle K DK Walch Michael M Martinvalet Denis D Thomas Marshall P MP Lieberman Judy J
Proceedings of the National Academy of Sciences of the United States of America 20120515 22
During immune-mediated death, death-inducing granzyme (Gzm) proteases concentrate in the nucleus of cells targeted for immune elimination, suggesting that nuclear processes are important targets. Here we used differential 2D proteomics of GzmA-treated nuclei to identify potential GzmA substrates. Of 44 candidates, 33 were RNA-binding proteins important in posttranscriptional RNA processing, including 14 heterogeneous nuclear ribonucleoproteins (hnRNP). Multiple hnRNPs were degraded in cells unde ...[more]