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?E-catenin is an autoinhibited molecule that coactivates vinculin.


ABSTRACT: ?E-catenin, an essential component of the adherens junction, interacts with the classical cadherin-?-catenin complex and with F-actin, but its precise role is unknown. ?E-catenin also binds to the F-actin-binding protein vinculin, which also appears to be important in junction assembly. Vinculin and ?E-catenin are homologs that contain a series of helical bundle domains, D1-D5. We mapped the vinculin-binding site to a sequence in D3a comprising the central two helices of a four-helix bundle. The crystal structure of this peptide motif bound to vinculin D1 shows that the two helices adopt a parallel, colinear arrangement suggesting that the ?E-catenin D3a bundle must unfold in order to bind vinculin. We show that ?E-catenin D3 binds strongly to vinculin, whereas larger fragments and full-length ?E-catenin bind approximately 1,000-fold more weakly. Thus, intramolecular interactions within ?E-catenin inhibit binding to vinculin. The actin-binding activity of vinculin is inhibited by an intramolecular interaction between the head (D1-D4) and the actin-binding D5 tail. In the absence of F-actin, there is no detectable binding of ?E-catenin D3 to full-length vinculin; however, ?E-catenin D3 promotes binding of vinculin to F-actin whereas full-length ?E-catenin does not. These findings support the combinatorial or "coincidence" model of activation in which binding of high-affinity proteins to the vinculin head and tail is required to shift the conformational equilibrium of vinculin from a closed, autoinhibited state to an open, stable F-actin-binding state. The data also imply that ?E-catenin must be activated in order to bind to vinculin.

SUBMITTER: Choi HJ 

PROVIDER: S-EPMC3365184 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

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αE-catenin is an autoinhibited molecule that coactivates vinculin.

Choi Hee-Jung HJ   Pokutta Sabine S   Cadwell Gregory W GW   Bobkov Andrey A AA   Bankston Laurie A LA   Liddington Robert C RC   Weis William I WI  

Proceedings of the National Academy of Sciences of the United States of America 20120514 22


αE-catenin, an essential component of the adherens junction, interacts with the classical cadherin-β-catenin complex and with F-actin, but its precise role is unknown. αE-catenin also binds to the F-actin-binding protein vinculin, which also appears to be important in junction assembly. Vinculin and αE-catenin are homologs that contain a series of helical bundle domains, D1-D5. We mapped the vinculin-binding site to a sequence in D3a comprising the central two helices of a four-helix bundle. The  ...[more]

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