Unknown

Dataset Information

0

Nucleostemin prevents telomere damage by promoting PML-IV recruitment to SUMOylated TRF1.


ABSTRACT: Continuously dividing cells must be protected from telomeric and nontelomeric DNA damage in order to maintain their proliferative potential. Here, we report a novel telomere-protecting mechanism regulated by nucleostemin (NS). NS depletion increased the number of telomere damage foci in both telomerase-active (TA(+)) and alternative lengthening of telomere (ALT) cells and decreased the percentage of damaged telomeres associated with ALT-associated PML bodies (APB) and the number of APB in ALT cells. Mechanistically, NS could promote the recruitment of PML-IV to SUMOylated TRF1 in TA(+) and ALT cells. This event was stimulated by DNA damage. Supporting the importance of NS and PML-IV in telomere protection, we demonstrate that loss of NS or PML-IV increased the frequency of telomere damage and aberration, reduced telomeric length, and perturbed the TRF2(?B?M)-induced telomeric recruitment of RAD51. Conversely, overexpression of either NS or PML-IV protected ALT and TA(+) cells from telomere damage. This work reveals a novel mechanism in telomere protection.

SUBMITTER: Hsu JK 

PROVIDER: S-EPMC3365494 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Nucleostemin prevents telomere damage by promoting PML-IV recruitment to SUMOylated TRF1.

Hsu Joseph K JK   Lin Tao T   Tsai Robert Y L RY  

The Journal of cell biology 20120501 5


Continuously dividing cells must be protected from telomeric and nontelomeric DNA damage in order to maintain their proliferative potential. Here, we report a novel telomere-protecting mechanism regulated by nucleostemin (NS). NS depletion increased the number of telomere damage foci in both telomerase-active (TA(+)) and alternative lengthening of telomere (ALT) cells and decreased the percentage of damaged telomeres associated with ALT-associated PML bodies (APB) and the number of APB in ALT ce  ...[more]

Similar Datasets

| S-EPMC1698521 | biostudies-literature
| S-EPMC9981704 | biostudies-literature
| S-EPMC1383544 | biostudies-literature
2023-08-19 | GSE240434 | GEO
| S-EPMC7826288 | biostudies-literature
| S-EPMC4050144 | biostudies-literature
| S-EPMC2806817 | biostudies-literature
| S-EPMC4777776 | biostudies-literature
| S-EPMC6802522 | biostudies-literature
| S-EPMC2648247 | biostudies-literature