Rendering protein-based particles transiently insoluble for therapeutic applications.
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ABSTRACT:
SUBMITTER: Xu J
PROVIDER: S-EPMC3365610 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Publications
Rendering protein-based particles transiently insoluble for therapeutic applications.
Xu Jing J Wang Jin J Luft J Christopher JC Tian Shaomin S Owens Gary G Pandya Ashish A AA Berglund Peter P Pohlhaus Patrick P Maynor Benjamin W BW Smith Jonathan J Hubby Bolyn B Napier Mary E ME DeSimone Joseph M JM
Journal of the American Chemical Society 20120517 21
Herein, we report the fabrication of protein (bovine serum albumin, BSA) particles which were rendered transiently insoluble using a novel, reductively labile disulfide-based cross-linker. After being cross-linked, the protein particles retain their integrity in aqueous solution and dissolve preferentially under a reducing environment. Our data demonstrates that cleavage of the cross-linker leaves no chemical residue on the reactive amino group. Delivery of a self-replicating RNA was achieved vi ...[more]
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