Ontology highlight
ABSTRACT:
SUBMITTER: Varma D
PROVIDER: S-EPMC3366049 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Varma Dileep D Chandrasekaran Srikripa S Sundin Lynsie J R LJ Reidy Karen T KT Wan Xiaohu X Chasse Dawn A D DA Nevis Kathleen R KR DeLuca Jennifer G JG Salmon E D ED Cook Jeanette Gowen JG
Nature cell biology 20120513 6
Cdt1, a protein critical for replication origin licensing in G1 phase, is degraded during S phase but re-accumulates in G2 phase. We now demonstrate that human Cdt1 has a separable essential mitotic function. Cdt1 localizes to kinetochores during mitosis through interaction with the Hec1 component of the Ndc80 complex. G2-specific depletion of Cdt1 arrests cells in late prometaphase owing to abnormally unstable kinetochore-microtubule (kMT) attachments and Mad1-dependent spindle-assembly-checkpo ...[more]