Ontology highlight
ABSTRACT:
SUBMITTER: Sapienza PJ
PROVIDER: S-EPMC3366113 | biostudies-literature | 2007 Nov
REPOSITORIES: biostudies-literature
Sapienza Paul J PJ Rosenberg John M JM Jen-Jacobson Linda L
Structure (London, England : 1993) 20071101 11
Promiscuous mutant EcoRI endonucleases bind to the canonical site GAATTC more tightly than does the wild-type endonuclease, yet cleave variant (EcoRI(*)) sites more rapidly than does wild-type. The crystal structure of the A138T promiscuous mutant homodimer in complex with a GAATTC site is nearly identical to that of the wild-type complex, except that the Thr138 side chains make packing interactions with bases in the 5'-flanking regions outside the recognition hexanucleotide while excluding two ...[more]