Ontology highlight
ABSTRACT:
SUBMITTER: Zhang Y
PROVIDER: S-EPMC3366836 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Zhang Yanhong Y Yan Wensheng W Jung Yong Sam YS Chen Xinbin X
The Journal of biological chemistry 20120328 21
p73 is expressed as TA and ΔN isoforms, both of which are implicated in tumor suppression and/or promotion. To address how p73 possesses these opposing functions, we developed three-dimensional culture of MCF10A cells, which undergo cell morphogenesis to form polarized spheroids with hollow lumen similar to normal mammary acini in vivo. Here, we showed that upon knockdown of p73, particularly TAp73 but not ΔNp73, MCF10A cells formed irregular and near-normal acini without hollow lumen in three-d ...[more]