Dataset Information

Bladder expression of CD cell surface antigens and cell-type-specific transcriptomes.

ABSTRACT: Many cell types have no known functional attributes. In the bladder and prostate, basal epithelial and stromal cells appear similar in cytomorphology and share several cell surface markers. Their total gene expression (transcriptome) should provide a clear measure of the extent to which they are alike functionally. Since urologic stromal cells are known to mediate organ-specific tissue formation, these cells in cancers might exhibit aberrant gene expression affecting their function. For transcriptomes, cluster designation (CD) antigens have been identified for cell sorting. The sorted cell populations can be analyzed by DNA microarrays. Various bladder cell types have unique complements of CD molecules. CD9(+) urothelial, CD104(+) basal and CD13(+) stromal cells of the lamina propria were therefore analyzed, as were CD9(+) cancer and CD13(+) cancer-associated stromal cells. The transcriptome datasets were compared by principal components analysis for relatedness between cell types; those with similarity in gene expression indicated similar function. Although bladder and prostate basal cells shared CD markers such as CD104, CD44 and CD49f, they differed in overall gene expression. Basal cells also lacked stem cell gene expression. The bladder luminal and stromal transcriptomes were distinct from their prostate counterparts. In bladder cancer, not only the urothelial but also the stromal cells showed gene expression alteration. The cancer process in both might thus involve defective stromal signaling. These cell-type transcriptomes provide a means to monitor in vitro models in which various CD-isolated cell types can be combined to study bladder differentiation and bladder tumor development based on cell-cell interaction.

SUBMITTER: Liu AY

PROVIDER: S-EPMC3367057 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Bladder expression of CD cell surface antigens and cell-type-specific transcriptomes.

Liu Alvin Y AY Vêncio Ricardo Z N RZ Page Laura S LS Ho Melissa E ME Loprieno Michelle A MA True Lawrence D LD

Cell and tissue research 20120320 3

Many cell types have no known functional attributes. In the bladder and prostate, basal epithelial and stromal cells appear similar in cytomorphology and share several cell surface markers. Their total gene expression (transcriptome) should provide a clear measure of the extent to which they are alike functionally. Since urologic stromal cells are known to mediate organ-specific tissue formation, these cells in cancers might exhibit aberrant gene expression affecting their function. For transcri ...[more]

PMID: 22427119

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Dataset Information

Bladder expression of CD cell surface antigens and cell-type-specific transcriptomes.

Publications

Bladder expression of CD cell surface antigens and cell-type-specific transcriptomes.

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