Ontology highlight
ABSTRACT:
SUBMITTER: Amin L
PROVIDER: S-EPMC3368133 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Amin Ladan L Ercolini Erika E Shahapure Rajesh R Migliorini Elisa E Torre Vincent V
Biophysical journal 20120605 11
We used optical tweezers to analyze the effect of jasplakinolide and cyclodextrin on the force exerted by lamellipodia from developing growth cones (GCs) of isolated dorsal root ganglia (DRG) neurons. We found that 25 nM of jasplakinolide, which is known to inhibit actin filament turnover, reduced both the maximal exerted force and maximal velocity during lamellipodia leading-edge protrusion. By using atomic force microscopy, we verified that cyclodextrin, which is known to remove cholesterol fr ...[more]