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A genomewide oscillation in transcription gates DNA replication and cell cycle.

ABSTRACT: Microarray analysis from a yeast continuous synchrony culture system shows a genomewide oscillation in transcription. Maximums in transcript levels occur at three nearly equally spaced intervals in this approximately 40-min cycle of respiration and reduction. Two temporal clusters (4,679 of 5,329) are maximally expressed during the reductive phase of the cycle, whereas a third cluster (650) is maximally expressed during the respiratory phase. Transcription is organized functionally into redox-state superclusters with genes known to be important in respiration or reduction being synthesized in opposite phases of the cycle. The transcriptional cycle gates synchronous bursts in DNA replication in a constant fraction of the population at 40-min intervals. Restriction of DNA synthesis to the reductive phase of the cycle may be an evolutionarily important mechanism for reducing oxidative damage to DNA during replication.

SUBMITTER: Klevecz RR

PROVIDER: S-EPMC337030 | biostudies-literature | 2004 Feb

REPOSITORIES: biostudies-literature

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A genomewide oscillation in transcription gates DNA replication and cell cycle.

Klevecz Robert R RR Bolen James J Forrest Gerald G Murray Douglas B DB

Proceedings of the National Academy of Sciences of the United States of America 20040120 5

Microarray analysis from a yeast continuous synchrony culture system shows a genomewide oscillation in transcription. Maximums in transcript levels occur at three nearly equally spaced intervals in this approximately 40-min cycle of respiration and reduction. Two temporal clusters (4,679 of 5,329) are maximally expressed during the reductive phase of the cycle, whereas a third cluster (650) is maximally expressed during the respiratory phase. Transcription is organized functionally into redox-st ...[more]

PMID: 14734811

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