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Aspergillus niger DLFCC-90 rhamnoside hydrolase, a new type of flavonoid glycoside hydrolase.


ABSTRACT: A novel rutin-?-L-rhamnosidase hydrolyzing ?-L-rhamnoside of rutin, naringin, and hesperidin was purified and characterized from Aspergillus niger DLFCC-90, and the gene encoding this enzyme, which is highly homologous to the ?-amylase gene, was cloned and expressed in Pichia pastoris GS115. The novel enzyme was classified in glycoside-hydrolase (GH) family 13.

SUBMITTER: Liu T 

PROVIDER: S-EPMC3370493 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Aspergillus niger DLFCC-90 rhamnoside hydrolase, a new type of flavonoid glycoside hydrolase.

Liu Tingqiang T   Yu Hongshan H   Zhang Chunzhi C   Lu Mingchun M   Piao Yongzhe Y   Ohba Masashi M   Tang Minqian M   Yuan Xiaodong X   Wei Shenghua S   Wang Kan K   Ma Anzhou A   Feng Xue X   Qin Siqing S   Mukai Chisato C   Tsuji Akira A   Jin Fengxie F  

Applied and environmental microbiology 20120427 13


A novel rutin-α-L-rhamnosidase hydrolyzing α-L-rhamnoside of rutin, naringin, and hesperidin was purified and characterized from Aspergillus niger DLFCC-90, and the gene encoding this enzyme, which is highly homologous to the α-amylase gene, was cloned and expressed in Pichia pastoris GS115. The novel enzyme was classified in glycoside-hydrolase (GH) family 13. ...[more]

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