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High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans.

ABSTRACT: We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.

SUBMITTER: Lee SF

PROVIDER: S-EPMC3370494 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans.

Lee Siu F SF White Vanessa L VL Weeks Andrew R AR Hoffmann Ary A AA Endersby Nancy M NM

Applied and environmental microbiology 20120420 13

We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening. ...[more]

PMID: 22522691

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