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Crystallization of the novel S-adenosyl-L-methionine-dependent C-methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysis.

ABSTRACT: Recombinant Q9F8T9 protein from Streptomyces rishiriensis (CouO), an S-adenosyl-L-methionine-dependent C-methyltransferase, has been successfully cloned, expressed and purified. CouO was crystallized from a single condition in the Morpheus crystallization screen. A vitrified crystal diffracted to 2.05 Å resolution and belonged to space group P2(1), with unit-cell parameters a = 33.02, b = 82.87, c = 76.77 Å, ? = 96.93°.

SUBMITTER: Lyskowski A

PROVIDER: S-EPMC3370914 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Crystallization of the novel S-adenosyl-L-methionine-dependent C-methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysis.

Lyskowski Andrzej A Tengg Martin M Steinkellner Georg G Schwab Helmut H Gruber-Khadjawi Mandana M Gruber Karl K

Acta crystallographica. Section F, Structural biology and crystallization communications 20120523 Pt 6

Recombinant Q9F8T9 protein from Streptomyces rishiriensis (CouO), an S-adenosyl-L-methionine-dependent C-methyltransferase, has been successfully cloned, expressed and purified. CouO was crystallized from a single condition in the Morpheus crystallization screen. A vitrified crystal diffracted to 2.05 Å resolution and belonged to space group P2(1), with unit-cell parameters a = 33.02, b = 82.87, c = 76.77 Å, β = 96.93°. ...[more]

PMID: 22684074

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