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On-chip continuous monitoring of motile microorganisms on an ePetri platform.

ABSTRACT: Self-imaging Petri dish platforms with microscopy resolution, which we term 'ePetri', can significantly streamline cell cultures and/or other longitudinal biological studies. In this paper, we demonstrate high-resolution imaging and long-term culture of motile microorganisms in a specialized ePetri platform by taking advantage of the inherent motion. By applying a super-resolution algorithm to a set of low-resolution images of the microorganisms as they move across the sensing area of a complementary metal oxide semiconductor (CMOS) image sensor chip, we can render an improved-resolution image of the microorganisms. We perform a longitudinal study of Euglena gracilis cultured in an ePetri platform, and image-based analysis on the motion and morphology of the cells. As a miniaturized and automated culture monitoring platform, this ePetri technology can greatly improve studies and experiments with motile microorganisms.

SUBMITTER: Lee SA

PROVIDER: S-EPMC3371133 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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On-chip continuous monitoring of motile microorganisms on an ePetri platform.

Lee Seung Ah SA Zheng Guoan G Mukherjee Nandini N Yang Changhuei C

Lab on a chip 20120420 13

Self-imaging Petri dish platforms with microscopy resolution, which we term 'ePetri', can significantly streamline cell cultures and/or other longitudinal biological studies. In this paper, we demonstrate high-resolution imaging and long-term culture of motile microorganisms in a specialized ePetri platform by taking advantage of the inherent motion. By applying a super-resolution algorithm to a set of low-resolution images of the microorganisms as they move across the sensing area of a compleme ...[more]

PMID: 22522781

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