Lipidomics of intact mitochondria by MALDI-TOF/MS.
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ABSTRACT:
SUBMITTER: Angelini R
PROVIDER: S-EPMC3371254 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Publications
Lipidomics of intact mitochondria by MALDI-TOF/MS.
Angelini Roberto R Vitale Rita R Patil Vinay A VA Cocco Tiziana T Ludwig Bernd B Greenberg Miriam L ML Corcelli Angela A
Journal of lipid research 20120503 7
A simple and fast method of lipid analysis of isolated intact mitochondria by means of MALDI-TOF mass spectrometry is described. Mitochondria isolated from bovine heart and yeast have been employed to set up and validate the new method of lipid analysis. The mitochondrial suspension is directly applied over the target and, after drying, covered by a thin layer of the 9-aminoacridine matrix solution. The lipid profiles acquired with this procedure contain all peaks previously obtained by analyzin ...[more]
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