Rapid identification of Sporothrix species by T3B fingerprinting.
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ABSTRACT:
SUBMITTER: de Oliveira MM
PROVIDER: S-EPMC3372112 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Publications
Rapid identification of Sporothrix species by T3B fingerprinting.
de Oliveira Manoel Marques Evangelista MM Sampaio Paula P Almeida-Paes Rodrigo R Pais Célia C Gutierrez-Galhardo Maria Clara MC Zancope-Oliveira Rosely Maria RM
Journal of clinical microbiology 20120307 6
This article describes PCR fingerprinting using the universal primer T3B to distinguish among species of the Sporothrix complex, S. brasiliensis, S. globosa, S. mexicana, and S. schenckii. This methodology generated distinct banding patterns, allowing the correct identification of all 35 clinical isolates at the species level, confirmed by partial calmodulin (CAL) gene sequence analyses. This methodology is simple, reliable, rapid, and cheap, making it an ideal routine identification system for ...[more]
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