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Standardized one-step real-time reverse transcription-PCR assay for universal detection and quantification of hepatitis delta virus from clinical samples in the presence of a heterologous internal-control RNA.

ABSTRACT: As for other chronic viral diseases, quantification of hepatitis delta virus (HDV) loads may be useful for patient management. We describe a one-step quantitative reverse transcription-PCR assay that is reliable and automatable and meets the regulatory authorities' standards for accurate quantification of the major HDV genotypes. It includes an internal control and uses in vitro-transcribed RNAs as standards. Its linearity range is 500 to 1.7 × 10(11) copies/ml, its sensitivity is around 150 copies/ml, its repeatability is around 15%, and its reproducibility is below 0.25 log(10) copies/ml.

SUBMITTER: Scholtes C

PROVIDER: S-EPMC3372137 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Standardized one-step real-time reverse transcription-PCR assay for universal detection and quantification of hepatitis delta virus from clinical samples in the presence of a heterologous internal-control RNA.

Scholtes Caroline C Icard Vinca V Amiri Majid M Chevallier-Queyron Philippe P Trabaud Mary-Anne MA Ramière Christophe C Zoulim Fabien F André Patrice P Dény Paul P

Journal of clinical microbiology 20120314 6

As for other chronic viral diseases, quantification of hepatitis delta virus (HDV) loads may be useful for patient management. We describe a one-step quantitative reverse transcription-PCR assay that is reliable and automatable and meets the regulatory authorities' standards for accurate quantification of the major HDV genotypes. It includes an internal control and uses in vitro-transcribed RNAs as standards. Its linearity range is 500 to 1.7 × 10(11) copies/ml, its sensitivity is around 150 cop ...[more]

PMID: 22422856

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