Ontology highlight
ABSTRACT:
SUBMITTER: Cisse II
PROVIDER: S-EPMC3372693 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Nature structural & molecular biology 20120513 6
Sequence recognition through base-pairing is essential for DNA repair and gene regulation, but the basic rules governing this process remain elusive. In particular, the kinetics of annealing between two imperfectly matched strands is not well characterized, despite its potential importance in nucleic acid-based biotechnologies and gene silencing. Here we use single-molecule fluorescence to visualize the multiple annealing and melting reactions of two untethered strands inside a porous vesicle, a ...[more]