Ontology highlight
ABSTRACT:
SUBMITTER: Li J
PROVIDER: S-EPMC3372940 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Li Jun J Witten Daniela M DM Johnstone Iain M IM Tibshirani Robert R
Biostatistics (Oxford, England) 20111014 3
We discuss the identification of genes that are associated with an outcome in RNA sequencing and other sequence-based comparative genomic experiments. RNA-sequencing data take the form of counts, so models based on the Gaussian distribution are unsuitable. Moreover, normalization is challenging because different sequencing experiments may generate quite different total numbers of reads. To overcome these difficulties, we use a log-linear model with a new approach to normalization. We derive a no ...[more]