Ontology highlight
ABSTRACT:
SUBMITTER: Seong J
PROVIDER: S-EPMC3373894 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Nature communications 20110726
Proper subcellular localization of focal adhesion kinase (FAK) is crucial for many cellular processes. It remains, however, unclear how FAK activity is regulated at subcellular compartments. To visualize the FAK activity at different membrane microdomains, we develop a fluorescence resonance energy transfer (FRET)-based FAK biosensor, and target it into or outside of detergent-resistant membrane (DRM) regions at the plasma membrane. Here we show that, on cell adhesion to extracellular matrix pro ...[more]