Dataset Information

Identification and classification of detoxification enzymes from Culex quinquefasciatus (Diptera: Culicidae).

ABSTRACT: Molecular characterization of the insecticide resistance has become a hot research topic ever since the first disease transmitting arthropod (Anopheles gambiae) genome sequence has unveiled in 2002. A recent publication of the Culex quinquefasciatus genome sequence has opened up new opportunities for molecular and comparative genomic analysis of multiple mosquito genomes to characterize the insecticide resistance. Here, we utilized a whole genome sequence of Cx. quinquefasciatus to identify putatively active members of the detoxification supergene families, namely cytochrome P450s (P450s), glutathione-S-transferases (GSTs), and choline/carboxylesterases (CCEs). The Culex genome analysis revealed 166 P450s, 40 GSTs, and 62 CCEs. Further, the comparative genomic analysis shows that these numbers are considerably higher than the other dipteran mosquitoes. These observed speciesspecific expansions of the detoxification super gene family members endorse the popular understanding of the involvement of these gene families in protecting the organism against multitudinous classes of toxic substances during its complex (aquatic and terrestrial) life cycle. Thus, the generated data set may provide an initial point to start with to characterize the insecticide resistance at a molecular level which could then lead the development of an easy to use molecular marker to monitor the incipient insecticide resistance in field environs.

SUBMITTER: Reddy BN

PROVIDER: S-EPMC3374374 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Identification and classification of detoxification enzymes from Culex quinquefasciatus (Diptera: Culicidae).

Reddy Bp Niranjan BN Rao B Prasad BP Prasad Gbks G Raghavendra K K

Bioinformation 20120515 9

Molecular characterization of the insecticide resistance has become a hot research topic ever since the first disease transmitting arthropod (Anopheles gambiae) genome sequence has unveiled in 2002. A recent publication of the Culex quinquefasciatus genome sequence has opened up new opportunities for molecular and comparative genomic analysis of multiple mosquito genomes to characterize the insecticide resistance. Here, we utilized a whole genome sequence of Cx. quinquefasciatus to identify puta ...[more]

PMID: 22715314

Similar Datasets

Project description:BACKGROUND: Culex spp. mosquitoes are considered to be the most important vectors of West Nile virus (WNV) detected in at least 34 species of mosquitoes in the United States. In North America, Culex pipiens pipiens, Culex pipiens quinquefasciatus, and Culex tarsalis are all competent vectors of WNV, which is considered to be enzootic in the United States and has also been detected in equines and birds in many states of Mexico and in humans in Nuevo Leon. There is potential for WNV to be introduced into Mexico City by various means including infected mosquitoes on airplanes, migrating birds, ground transportation and infected humans. Little is known of the geographic distribution of Culex pipiens complex mosquitoes and hybrids in Mexico City. Culex pipiens pipiens preferentially feed on avian hosts; Culex pipiens quinquefasciatus have historically been considered to prefer mammalian hosts; and hybrids of these two species could theoretically serve as bridge vectors to transmit WNV from avian hosts to humans and other mammalian hosts. In order to address the potential of WNV being introduced into Mexico City, we have determined the identity and spatial distribution of Culex pipiens complex mosquitoes and their hybrids. RESULTS: Mosquito larvae collected from 103 sites throughout Mexico City during 2004-2005 were identified as Culex, Culiseta or Ochlerotatus by morphological analysis. Within the genus Culex, specimens were further identified as Culex tarsalis or as belonging to the Culex pipiens complex. Members of the Culex pipiens complex were separated by measuring the ratio of the dorsal and ventral arms (DV/D ratio) of the male genitalia and also by using diagnostic primers designed for the Ace.2 gene. Culex pipiens quinquefasciatus was the most abundant form collected. CONCLUSIONS: Important WNV vectors species, Cx. p. pipiens, Cx. p. quinquefasciatus and Cx. tarsalis, are all present in Mexico City. Hybrids of Cx. p. pipiens and Cx. p. quinquefasciatus were also collected and identified. The presence and abundance of these WNV competent vectors is a cause for concern. Understanding the distribution of these vectors can help improve viral surveillance activities and mosquito control efforts in Mexico City.

Project description:BackgroundWater quality is known to influence the development and survival of larval mosquitoes, which affects mosquito-borne pathogen transmission as a function of the number of mosquitoes that reach adulthood and blood feed. Although water properties are known to affect mosquito development, few studies have investigated the link among soil properties, water quality, and mosquito development. Given the large number of ground-breeding mosquito species, this linkage is a potentially important factor to consider in mosquito ecology. In this study, we explored the effects of different soils on multiple life history parameters of the ground-breeding mosquito species Culex quinquefasciatus (Diptera: Culicidae).MethodsCx. quinquefasciatus larvae were reared in water combined with different soil substrates (sandy, silt, or clay loam textures) at increasing soil to water volume ratios, with and without the addition of organic matter (fish food). Gravid mosquitoes were offered different soil-water extracts to investigate soil effects on oviposition preference.ResultsWithout the addition of organic matter, larval survival and development differed significantly among waters with different soil textures and volumes of substrate. Mosquitoes in water with clay loam soil survived longer and developed further than mosquitoes in other soil waters. Larvae survived for longer periods of time with increased volumes of soil substrate. Adding organic matter reduced the differences in larval survival time, development, and pupation among soil-water extracts. Adult female mosquitoes oviposited more frequently in water with clay loam soil, but the addition of organic matter reduced the soil effects on oviposition preference.ConclusionsThis study suggests soil composition affects larval mosquito survival and development, as well as the oviposition preference of gravid females. Future studies could differentiate abiotic and biotic soil features that affect mosquitoes and incorporate soil variation at the landscape scale into models to predict mosquito population dynamics and mosquito-borne pathogen transmission.

Dataset Information

Identification and classification of detoxification enzymes from Culex quinquefasciatus (Diptera: Culicidae).

Publications

Identification and classification of detoxification enzymes from Culex quinquefasciatus (Diptera: Culicidae).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets