Ontology highlight
ABSTRACT:
SUBMITTER: Han AR
PROVIDER: S-EPMC3374512 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Han Ah Reum AR Kim Hyun Sook HS Cho Gye Yoon GY Ki Ho Sam HS Kim Hwa Young HY Hwang Kwang Yeon KY
Acta crystallographica. Section F, Structural biology and crystallization communications 20120420 Pt 5
Methionine sulfoxide reductase (Msr) is a repair enzyme that reduces oxidized methionine to methionine. The Msr enzyme is divided into MsrA and MsrB, which reduce the S and R configurations of the substrate, respectively. In some pathogenic bacteria MsrA and MsrB exist in a fusion-protein form, MsrAB. In this study, the recombinant MsrA part of MsrAB from Haemophilus influenzae (HIMsrA) was overexpressed, purified and crystallized using the hanging-drop vapour-diffusion method. A diffraction dat ...[more]