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Expression, purification and crystallization of the Crimean-Congo haemorrhagic fever virus nucleocapsid protein.

ABSTRACT: Crimean-Congo haemorrhagic fever virus (CCHFV) is a member of the Nairovirus genus within the Bunyaviridae family of segmented negative-sense RNA viruses. This paper describes the expression, purification and crystallization of full-length CCHFV nucleocapsid (N) protein and the collection of a 2.1?Å resolution X-ray diffraction data set using synchrotron radiation. Crystals of the CCHFV N protein belonged to space group C2, with unit-cell parameters a = 150.38, b = 72.06, c = 101.23?Å, ? = 110.70° and two molecules in the asymmetric unit. Circular-dichroism analysis provided insight into the secondary structure, whilst gel-filtration analysis revealed possible oligomeric states of the N protein. Structural determination is ongoing.

SUBMITTER: Carter SD

PROVIDER: S-EPMC3374515 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

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Expression, purification and crystallization of the Crimean-Congo haemorrhagic fever virus nucleocapsid protein.

Carter S D SD Barr J N JN Edwards T A TA

Acta crystallographica. Section F, Structural biology and crystallization communications 20120421 Pt 5

Crimean-Congo haemorrhagic fever virus (CCHFV) is a member of the Nairovirus genus within the Bunyaviridae family of segmented negative-sense RNA viruses. This paper describes the expression, purification and crystallization of full-length CCHFV nucleocapsid (N) protein and the collection of a 2.1 Å resolution X-ray diffraction data set using synchrotron radiation. Crystals of the CCHFV N protein belonged to space group C2, with unit-cell parameters a = 150.38, b = 72.06, c = 101.23 Å, β = 110.7 ...[more]

PMID: 22691790

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