Ontology highlight
ABSTRACT:
SUBMITTER: Hirai H
PROVIDER: S-EPMC3374774 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Hirai Hiroyuki H Firpo Meri M Kikyo Nobuaki N
PloS one 20120613 6
Human induced pluripotent stem cells (iPSCs) can be divided into a leukemia inhibitory factor (LIF)-dependent naïve type and a basic fibroblast growth factor (bFGF)-dependent primed type. Although the former are more undifferentiated than the latter, they require signal transduction inhibitors and sustained expression of the transgenes used for iPSC production. We used a transcriptionally enhanced version of OCT4 to establish LIF-dependent human iPSCs without the use of inhibitors and sustained ...[more]