Ontology highlight
ABSTRACT:
SUBMITTER: Lancaster KM
PROVIDER: S-EPMC3375907 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20101001 41
Redox and spectroscopic (electronic absorption, multifrequency electron paramagnetic resonance (EPR), and X-ray absorption) properties together with X-ray crystal structures are reported for the type 2 Cu(II) C112D/M121E variant of Pseudomonas aeruginosa azurin. The results suggest that Cu(II) is constrained from interaction with the proximal glutamate; this structural frustration implies a "rack" mechanism for the 290 mV (vs NHE) reduction potential measured at neutral pH. At high pH (∼9), hydr ...[more]