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Selective fluorescence detection of cysteine and N-terminal cysteine peptide residues.

ABSTRACT: A new fluorogenic fluorescein derivative containing an alpha,beta-unsaturated aldehyde moiety produces a selective fluorescent signal enhancement in the presence of cysteine or peptides containing N-terminal cysteine residues. The mechanism is based on synergistic covalent and supramolecular interactions.

SUBMITTER: Lim S

PROVIDER: S-EPMC3376164 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature

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Selective fluorescence detection of cysteine and N-terminal cysteine peptide residues.

Lim Soojin S Escobedo Jorge O JO Lowry Mark M Xu Xiangyang X Strongin Robert R

Chemical communications (Cambridge, England) 20100708 31

A new fluorogenic fluorescein derivative containing an alpha,beta-unsaturated aldehyde moiety produces a selective fluorescent signal enhancement in the presence of cysteine or peptides containing N-terminal cysteine residues. The mechanism is based on synergistic covalent and supramolecular interactions. ...[more]

PMID: 20617237

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