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Visual detection of cysteine and homocysteine.


ABSTRACT: The determination of cysteine and homocysteine levels is of great current interest for the monitoring of desease states. A new colorimetric method for the simultaneous detection of l-cysteine and l-homocysteine has been developed. A fluorescein derivative reacts with the above amino acids, producing their respective thiazolidines resulting in color changes. Interference from other amino acids and proteins is minimal.

SUBMITTER: Rusin O 

PROVIDER: S-EPMC3376414 | biostudies-literature | 2004 Jan

REPOSITORIES: biostudies-literature

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Visual detection of cysteine and homocysteine.

Rusin Oleksandr O   St Luce Nadia N NN   Agbaria Rezik A RA   Escobedo Jorge O JO   Jiang Shan S   Warner Isiah M IM   Dawan Fareed B FB   Lian Kun K   Strongin Robert M RM  

Journal of the American Chemical Society 20040101 2


The determination of cysteine and homocysteine levels is of great current interest for the monitoring of desease states. A new colorimetric method for the simultaneous detection of l-cysteine and l-homocysteine has been developed. A fluorescein derivative reacts with the above amino acids, producing their respective thiazolidines resulting in color changes. Interference from other amino acids and proteins is minimal. ...[more]

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