Ontology highlight
ABSTRACT:
SUBMITTER: Lesoine JF
PROVIDER: S-EPMC3377747 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Lesoine John F JF Venkataraman Prahnesh A PA Maloney Peter C PC Dumont Mark E ME Novotny Lukas L
Nano letters 20120604 6
We present a method for measuring the fluorescence from a single molecule hundreds of times without surface immobilization. The approach is based on the use of electroosmosis to repeatedly drive a single target molecule in a fused silica nanochannel through a stationary laser focus. Single molecule fluorescence detected during the transit time through the laser focus is used to repeatedly reverse the electrical potential controlling the flow direction. Our method does not rely on continuous obse ...[more]