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Identification of Enzyme Inhibitors Using Therapeutic Target Protein - Magnetic Nanoparticle Conjugates.

ABSTRACT: Target protein - magnetic nanoparticle (MNP) conjugates, i.e. ?-glucosidase-MNP and protein tyrosine phosphatase 1B (PTP1B)-MNP, were prepared and evaluated for the first time for affinity extraction of the enzyme inhibitors from herbal extracts. Four ligands extracted from granati pericarpium were identified by ESI-MS analysis. In vitro tests indicated that they inhibited both ?-glucosidase and PTP1B, two important target proteins for diabetic treatment.

SUBMITTER: Qing LS

PROVIDER: S-EPMC3378064 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature

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Identification of Enzyme Inhibitors Using Therapeutic Target Protein - Magnetic Nanoparticle Conjugates.

Qing Lin-Sen LS Tang Nan N Xue Ying Y Liang Jian J Liu Yi-Ming YM Liao Xun X

Analytical methods : advancing methods and applications 20120101 6

Target protein - magnetic nanoparticle (MNP) conjugates, i.e. α-glucosidase-MNP and protein tyrosine phosphatase 1B (PTP1B)-MNP, were prepared and evaluated for the first time for affinity extraction of the enzyme inhibitors from herbal extracts. Four ligands extracted from granati pericarpium were identified by ESI-MS analysis. In vitro tests indicated that they inhibited both α-glucosidase and PTP1B, two important target proteins for diabetic treatment. ...[more]

PMID: 22737184

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