Dataset Information

Upregulation of miR-196b confers a poor prognosis in glioblastoma patients via inducing a proliferative phenotype.

ABSTRACT:

Purpose

To explore the expression pattern, prognostic value and functional role of miR-196b in glioblastoma (GBM) patients using large cohorts.

Experimental design

MiR-196b expression was measured using the Human v2.0 miRNA Expression BeadChip (Illumina) in 198 frozen glioma tissues. The expression levels of miR-196b were also validated in an independent cohort containing 128 formalin-fixed paraffin-embedded (FFPE) glioma samples using qRT-PCR. The presence of other molecular prognostic indicators was assessed centrally in the glioma samples. Whole genome gene profiling was performed to investigate the underlying biological behavior. MiR-196b functional analyses were performed in U87 and U251 cell lines.

Results

The expression levels of miR-196b were inversely correlated with overall survival in GBM patients. Gene set enrichment analysis (GSEA) showed that the gene sets relating to cell cycle were significantly enriched in the cases with miR-196b overexpression. Functional analyses in U87 and U251 cells revealed that miR-196b was involved in cell proliferation.

Conclusions

MiR-196b is overexpressed and confers a poor prognosis via promoting cellular proliferation in GBM patients.

SUBMITTER: Ma R

PROVIDER: S-EPMC3378534 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Publications

Upregulation of miR-196b confers a poor prognosis in glioblastoma patients via inducing a proliferative phenotype.

Ma Ruimin R Yan Wei W Zhang Guojun G Lv Hong H Liu Zhizhong Z Fang Fang F Zhang Wei W Zhang Junxia J Tao Tao T You Yongping Y Jiang Tao T Kang Xixiong X

PloS one 20120619 6

<h4>Purpose</h4>To explore the expression pattern, prognostic value and functional role of miR-196b in glioblastoma (GBM) patients using large cohorts.<h4>Experimental design</h4>MiR-196b expression was measured using the Human v2.0 miRNA Expression BeadChip (Illumina) in 198 frozen glioma tissues. The expression levels of miR-196b were also validated in an independent cohort containing 128 formalin-fixed paraffin-embedded (FFPE) glioma samples using qRT-PCR. The presence of other molecular prog ...[more]

PMID: 22723849

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Dataset Information

Upregulation of miR-196b confers a poor prognosis in glioblastoma patients via inducing a proliferative phenotype.

Purpose

Experimental design

Results

Conclusions

Publications

Upregulation of miR-196b confers a poor prognosis in glioblastoma patients via inducing a proliferative phenotype.

Similar Datasets

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