Ontology highlight
ABSTRACT:
SUBMITTER: Sholukh AM
PROVIDER: S-EPMC3380854 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Sholukh Anton M AM Mukhtar Muhammad M MM Humbert Michael M Essono Sosthène S SS Watkins Jennifer D JD Vyas Hemant K HK Shanmuganathan Vivekanandan V Hemashettar Girish G Kahn Maria M Hu Shiu-Lok SL Montefiori David C DC Polonis Victoria R VR Schur Peter H PH Ruprecht Ruth M RM
PloS one 20120621 6
Existing technologies allow isolating antigen-specific monoclonal antibodies (mAbs) from B cells. We devised a direct approach to isolate mAbs with predetermined conformational epitope specificity, using epitope mimetics (mimotopes) that reflect the three-dimensional structure of given antigen subdomains. We performed differential biopanning using bacteriophages encoding random peptide libraries and polyclonal antibodies (Abs) that had been affinity-purified with either native or denatured antig ...[more]