Unknown

Dataset Information

0

Attachment of Chlamydia trachomatis L2 to host cells requires sulfation.


ABSTRACT: Chlamydia trachomatis is a pathogen responsible for a prevalent sexually transmitted disease. It is also the most common cause of infectious blindness in the developing world. We performed a loss-of-function genetic screen in human haploid cells to identify host factors important in C. trachomatis L2 infection. We identified and confirmed B3GAT3, B4GALT7, and SLC35B2, which encode glucuronosyltransferase I, galactosyltransferase I, and the 3'-phosphoadenosine 5'-phosphosulfate transporter 1, respectively, as important in facilitating Chlamydia infection. Knockout of any of these three genes inhibits Chlamydia attachment. In complementation studies, we found that the introduction of functional copies of these three genes into the null clones restored full susceptibility to Chlamydia infection. The degree of attachment of Chlamydia strongly correlates with the level of sulfation of the host cell, not simply with the amount of heparan sulfate. Thus, other, as-yet unidentified sulfated macromolecules must contribute to infection. These results demonstrate the utility of screens in haploid cells to study interactions of human cells with bacteria. Furthermore, the human null clones generated can be used to investigate the role of heparan sulfate and sulfation in other settings not limited to infectious disease.

SUBMITTER: Rosmarin DM 

PROVIDER: S-EPMC3382535 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Attachment of Chlamydia trachomatis L2 to host cells requires sulfation.

Rosmarin David M DM   Carette Jan E JE   Olive Andrew J AJ   Starnbach Michael N MN   Brummelkamp Thijn R TR   Ploegh Hidde L HL  

Proceedings of the National Academy of Sciences of the United States of America 20120606 25


Chlamydia trachomatis is a pathogen responsible for a prevalent sexually transmitted disease. It is also the most common cause of infectious blindness in the developing world. We performed a loss-of-function genetic screen in human haploid cells to identify host factors important in C. trachomatis L2 infection. We identified and confirmed B3GAT3, B4GALT7, and SLC35B2, which encode glucuronosyltransferase I, galactosyltransferase I, and the 3'-phosphoadenosine 5'-phosphosulfate transporter 1, res  ...[more]

Similar Datasets

2023-11-29 | GSE234589 | GEO
| S-EPMC2774160 | biostudies-other
| PRJEA71105 | ENA
| PRJNA1200737 | ENA
| S-EPMC3104491 | biostudies-literature
| S-EPMC5400845 | biostudies-literature
| S-EPMC4796813 | biostudies-other
| S-EPMC9199516 | biostudies-literature
| PRJNA66013 | ENA
| PRJNA298309 | ENA