Ebselen and congeners inhibit NADPH oxidase 2-dependent superoxide generation by interrupting the binding of regulatory subunits.
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ABSTRACT:
SUBMITTER: Smith SM
PROVIDER: S-EPMC3383625 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
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Ebselen and congeners inhibit NADPH oxidase 2-dependent superoxide generation by interrupting the binding of regulatory subunits.
Smith Susan M E SM Min Jaeki J Ganesh Thota T Diebold Becky B Kawahara Tsukasa T Zhu Yerun Y McCoy James J Sun Aiming A Snyder James P JP Fu Haian H Du Yuhong Y Lewis Iestyn I Lambeth J David JD
Chemistry & biology 20120601 6
NADPH oxidases (Nox) are a primary source of reactive oxygen species (ROS), which function in normal physiology and, when overproduced, in pathophysiology. Recent studies using mice deficient in Nox2 identify this isoform as a novel target against Nox2-implicated inflammatory diseases. Nox2 activation depends on the binding of the proline-rich domain of its heterodimeric partner p22phox to p47phox. A high-throughput screen that monitored this interaction via fluorescence polarization identified ...[more]
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