Ontology highlight
ABSTRACT:
SUBMITTER: Kirby LA
PROVIDER: S-EPMC3383737 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Kirby Leslie A LA Schott Jason T JT Noble Brenda L BL Mendez Daniel C DC Caseley Paul S PS Peterson Sarah C SC Routledge Tyler J TJ Patel Nilay V NV
PloS one 20120626 6
Canonical Wnt/β-catenin signaling has been suggested to promote self-renewal of pluripotent mouse and human embryonic stem cells. Here, we show that SB-216763, a glycogen synthase kinase-3 (GSK3) inhibitor, can maintain mouse embryonic stem cells (mESCs) in a pluripotent state in the absence of exogenous leukemia inhibitory factor (LIF) when cultured on mouse embryonic fibroblasts (MEFs). MESCs maintained with SB-216763 for one month were morphologically indistinguishable from LIF-treated mESCs ...[more]