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CD36 in the periphery and brain synergizes in stroke injury in hyperlipidemia.


ABSTRACT: Hyperlipidemia exacerbates ischemic stroke outcome and increases CD36 expression in the postischemic brain as well as in peripheral monocytes/macrophages. By exchanging bone marrow-derived cells between CD36-expressing and CD36-deficient mice, this study investigates the contribution of peripheral CD36 in comparison with that of brain CD36 to stroke pathology in hyperlipidemia.Following bone marrow transplantation, mice were fed a high-fat diet for 11 weeks and then subjected to ischemic stroke. Stroke outcome, expression of brain CD36, monocyte chemoattractant protein-1 (MCP-1), CCR2, and plasma MCP-1 levels were determined at 3 days postischemia. CD36 and CCR2 expression were also determined in splenocytes incubated with serum obtained from CD36-expressing or CD36-deficient mice.Infiltrating immune cells from the periphery are the major source of CD36 in the postischemic brain and contribute to stroke-induced brain injury. This CD36 effect was dependent on the modulation of MCP-1 and CCR2 expression in peripheral immune cells as well as CD36-expressing cells in the host brain.This study demonstrates that CD36 expressed in the periphery and brain synergize in ischemic brain injury through regulation of the MCP-1/CCR2 chemokine axis in hyperlipidemic conditions.

SUBMITTER: Kim E 

PROVIDER: S-EPMC3383818 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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CD36 in the periphery and brain synergizes in stroke injury in hyperlipidemia.

Kim Eunhee E   Febbraio Maria M   Bao Yi Y   Tolhurst Aaron T AT   Epstein Jeffrey M JM   Cho Sunghee S  

Annals of neurology 20120601 6


<h4>Objective</h4>Hyperlipidemia exacerbates ischemic stroke outcome and increases CD36 expression in the postischemic brain as well as in peripheral monocytes/macrophages. By exchanging bone marrow-derived cells between CD36-expressing and CD36-deficient mice, this study investigates the contribution of peripheral CD36 in comparison with that of brain CD36 to stroke pathology in hyperlipidemia.<h4>Methods</h4>Following bone marrow transplantation, mice were fed a high-fat diet for 11 weeks and  ...[more]

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