Ontology highlight
ABSTRACT:
SUBMITTER: Sandoval NR
PROVIDER: S-EPMC3387050 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Sandoval Nicholas R NR Kim Jaoon Y H JY Glebes Tirzah Y TY Reeder Philippa J PJ Aucoin Hanna R HR Warner Joseph R JR Gill Ryan T RT
Proceedings of the National Academy of Sciences of the United States of America 20120611 26
We describe a directed genome-engineering approach that combines genome-wide methods for mapping genes to traits [Warner JR, Reeder PJ, Karimpour-Fard A, Woodruff LBA, Gill RT (2010) Nat Biotechnol 28:856-862] with strategies for rapidly creating combinatorial ribosomal binding site (RBS) mutation libraries containing billions of targeted modifications [Wang HH, et al. (2009) Nature 460:894-898]. This approach should prove broadly applicable to various efforts focused on improving production of ...[more]