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An IFN-?-stimulated ATF6-C/EBP-?-signaling pathway critical for the expression of Death Associated Protein Kinase 1 and induction of autophagy.


ABSTRACT: The IFN family of cytokines operates a frontline defense against pathogens and neoplastic cells in vivo by controlling the expression of several genes. The death-associated protein kinase 1 (DAPK1), an IFN-?-induced enzyme, controls cell cycle, apoptosis, autophagy, and tumor metastasis, and its expression is frequently down-regulated in a number of human tumors. Although the biochemical action of DAPK1 is well understood, mechanisms that regulate its expression are unclear. Previously, we have shown that transcription factor C/EBP-? is required for the basal and IFN-?-induced expression of DAPK1. Here, we show that ATF6, an ER stress-induced transcription factor, interacts with C/EBP-? in an IFN-stimulated manner and is obligatory for Dapk1 expression. IFN-stimulated proteolytic processing of ATF6 and ERK1/2-mediated phosphorylation of C/EBP-? are necessary for these interactions. More importantly, IFN-? failed to activate autophagic response in cells lacking either ATF6 or C/EBP-?. Consistent with these observations, the Atf6(-/-) mice were highly susceptible to lethal bacterial infections compared with the wild-type mice. These studies not only unravel an IFN signaling pathway that controls cell growth and antibacterial defense, but also expand the role of ATF6 beyond ER stress.

SUBMITTER: Gade P 

PROVIDER: S-EPMC3387052 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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An IFN-γ-stimulated ATF6-C/EBP-β-signaling pathway critical for the expression of Death Associated Protein Kinase 1 and induction of autophagy.

Gade Padmaja P   Ramachandran Girish G   Maachani Uday B UB   Rizzo Mark A MA   Okada Tetsuya T   Prywes Ron R   Cross Alan S AS   Mori Kazutoshi K   Kalvakolanu Dhananjaya V DV  

Proceedings of the National Academy of Sciences of the United States of America 20120613 26


The IFN family of cytokines operates a frontline defense against pathogens and neoplastic cells in vivo by controlling the expression of several genes. The death-associated protein kinase 1 (DAPK1), an IFN-γ-induced enzyme, controls cell cycle, apoptosis, autophagy, and tumor metastasis, and its expression is frequently down-regulated in a number of human tumors. Although the biochemical action of DAPK1 is well understood, mechanisms that regulate its expression are unclear. Previously, we have  ...[more]

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