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Metabolite profiling and quantitative genetics of natural variation for flavonoids in Arabidopsis.

ABSTRACT: Little is known about the range and the genetic bases of naturally occurring variation for flavonoids. Using Arabidopsis thaliana seed as a model, the flavonoid content of 41 accessions and two recombinant inbred line (RIL) sets derived from divergent accessions (Cvi-0×Col-0 and Bay-0×Shahdara) were analysed. These accessions and RILs showed mainly quantitative rather than qualitative changes. To dissect the genetic architecture underlying these differences, a quantitative trait locus (QTL) analysis was performed on the two segregating populations. Twenty-two flavonoid QTLs were detected that accounted for 11-64% of the observed trait variations, only one QTL being common to both RIL sets. Sixteen of these QTLs were confirmed and coarsely mapped using heterogeneous inbred families (HIFs). Three genes, namely TRANSPARENT TESTA (TT)7, TT15, and MYB12, were proposed to underlie their variations since the corresponding mutants and QTLs displayed similar specific flavonoid changes. Interestingly, most loci did not co-localize with any gene known to be involved in flavonoid metabolism. This latter result shows that novel functions have yet to be characterized and paves the way for their isolation.

SUBMITTER: Routaboul JM

PROVIDER: S-EPMC3388840 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Metabolite profiling and quantitative genetics of natural variation for flavonoids in Arabidopsis.

Routaboul Jean-Marc JM Dubos Christian C Beck Gilles G Marquis Catherine C Bidzinski Przemyslaw P Loudet Olivier O Lepiniec Loïc L

Journal of experimental botany 20120322 10

Little is known about the range and the genetic bases of naturally occurring variation for flavonoids. Using Arabidopsis thaliana seed as a model, the flavonoid content of 41 accessions and two recombinant inbred line (RIL) sets derived from divergent accessions (Cvi-0×Col-0 and Bay-0×Shahdara) were analysed. These accessions and RILs showed mainly quantitative rather than qualitative changes. To dissect the genetic architecture underlying these differences, a quantitative trait locus (QTL) anal ...[more]

PMID: 22442426

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Dataset Information

Metabolite profiling and quantitative genetics of natural variation for flavonoids in Arabidopsis.

Publications

Metabolite profiling and quantitative genetics of natural variation for flavonoids in Arabidopsis.

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