Unknown

Dataset Information

0

Inflammation-associated regulation of the macrophage inhibitory cytokine (MIC-1) gene in prostate cancer.


ABSTRACT: Macrophage inhibitory cytokine-1 (MIC-1), also known as prostate-derived factor (PDF), is a molecule of the TGF-? superfamily and has been associated with the progression of various types of diseases including prostate cancer. Initially identified from activated macrophages, the MIC-1 gene may provide a potential link between inflammation and prostate cancer. In this context, we performed MIC-1 expression analysis using mouse prostate tissues to determine whether there was any correlation with age and inflammation. Reverse transcription PCR analysis on RNA samples isolated from prostate lobes from prostate-specific antigen transgenic mice of varying ages revealed that MIC-1 gene expression is extremely low to non-detectable in the prostate tissues obtained from young mice, while its expression increases in the prostate tissues harvested from elderly mice. Increased MIC-1 gene expression in the mouse prostate was found to be associated with an increased level of infiltrating lymphocytes. To confirm this observation, we showed that inflammation-associated cytokines (IL-1? and TNF-?) significantly upregulate the secretion of the MIC-1 protein in a human prostate cancer cell line (LNCaP cells), while cytokines IL-6 and granulocyte macrophage colony-stimulating factor were less effective. Taken together, these data indicated that inflammation-associated cytokines may play a critical role in the functional regulation of the MIC-1 gene in the early stages of prostate cancer development. More studies are required to understand the biological activity of MIC-1 gene regulation in the development and progression of prostate cancer.

SUBMITTER: Dubey S 

PROVIDER: S-EPMC3389684 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Inflammation-associated regulation of the macrophage inhibitory cytokine (MIC-1) gene in prostate cancer.

Dubey Seema S   Vanveldhuizen Peter P   Holzbeierlein Jeffrey J   Tawfik Ossama O   Thrasher J Brantley JB   Karan Dev D  

Oncology letters 20120306 5


Macrophage inhibitory cytokine-1 (MIC-1), also known as prostate-derived factor (PDF), is a molecule of the TGF-β superfamily and has been associated with the progression of various types of diseases including prostate cancer. Initially identified from activated macrophages, the MIC-1 gene may provide a potential link between inflammation and prostate cancer. In this context, we performed MIC-1 expression analysis using mouse prostate tissues to determine whether there was any correlation with a  ...[more]

Similar Datasets

| S-EPMC2376360 | biostudies-other
| S-EPMC3557964 | biostudies-literature
| S-EPMC23523 | biostudies-literature
| S-EPMC2896817 | biostudies-literature
| S-EPMC8118591 | biostudies-literature
| S-EPMC4390224 | biostudies-literature
| S-EPMC3619053 | biostudies-literature
| S-EPMC4562638 | biostudies-literature
| S-EPMC5966362 | biostudies-literature
2023-06-06 | MSV000092110 | MassIVE