Ontology highlight
ABSTRACT:
SUBMITTER: Ly M
PROVIDER: S-EPMC3390146 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Analytical and bioanalytical chemistry 20100421 2
Heparosan is the key precursor for the preparation of bioengineered heparin, a potential replacement for porcine intestinal heparin, an important anticoagulant drug. The molecular weight (MW) distribution of heparosan produced by the fermentation of E. coli K5 was investigated. Large-slab isocratic and mini-slab gradient polyacrylamide gel electrophoresis (PAGE) were used to analyze the MW and polydispersity of heparosan. A preparative method that allowed fractionation by continuous-elution PAGE ...[more]