Ontology highlight
ABSTRACT:
SUBMITTER: Horiuchi M
PROVIDER: S-EPMC3390167 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Horiuchi Masataka M Takahasi Kiyohiro K Kobashigawa Yoshihiro Y Ochiai Masanori M Inagaki Fuyuhiko F
Protein engineering, design & selection : PEDS 20120615 8
Silkworm β-1,3-glucan recognition protein (βGRP) tightly and specifically associates with β-1,3-glucan. We report here an affinity purification system named the 'GRP system', which uses the association between the β-1,3-glucan recognition domain of βGRP (GRP-tag), as an affinity tag, and curdlan beads. Curdlan is a water-insoluble β-1,3-glucan reagent, the low cost of which (about 100 JPY/g) allows the economical preparation of beads. Curdlan beads can be readily prepared by solubilization in an ...[more]