Self-calibrating viscosity probes: design and subcellular localization.
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ABSTRACT:
SUBMITTER: Dakanali M
PROVIDER: S-EPMC3390230 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Publications
Self-calibrating viscosity probes: design and subcellular localization.
Dakanali Marianna M Do Thai H TH Horn Austin A Chongchivivat Akaraphon A Jarusreni Tuptim T Lichlyter Darcy D Guizzunti Gianni G Haidekker Mark A MA Theodorakis Emmanuel A EA
Bioorganic & medicinal chemistry 20120519 14
We describe the design, synthesis and fluorescence profiles of new self-calibrating viscosity dyes in which a coumarin (reference fluorophore) has been covalently linked with a molecular rotor (viscosity sensor). Characterization of their fluorescence properties was made with separate excitation of the units and through resonance energy transfer from the reference to the sensor dye. We have modified the linker and the substitution of the rotor in order to change the hydrophilicity of these probe ...[more]
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